Flow cytometry resources

Flow cytometry resources

Researcher: 

Flow cytometry resources

The institute’s flow cytometry laboratory is one of the best equipped in Australia. We operate seven cell sorters and 10 analytical instruments. Our instruments have up to five lasers with up to 18 fluorescence detectors.

Our instruments are available for external as well as internal use. Experienced laboratory staff operate cell sorters, and analysers are operated by investigators. Training can be provided for all analytical instruments; flow laboratory staff can also prepare and analyse samples by arrangement.

Contact laboratory head Simon Monard, email monard@wehi.edu.au or phone +61 3 9345 2540 for further information.

Instrumentation

Cell Sorters

AriaW               5 Laser, 375nm, 405nm, 488nm, 561nm and 640nm

AriaC                4 laser, 375nm, 405nm, 488nm, and 640nm

Aria III              4 laser 405nm, 488nm, 561nm and 640nm

Aria L               4 laser, 375nm, 405nm, 488nm, and 640nm

Aria Fusion      4 laser 405nm, 488nm, 561nm and 640nm

Influx                5 Laser, 355nm, 405nm, 488nm, 561nm and 640nm

Moflo               3 lasers 488nm, 561nm and 640nm

Analysers

Fortessa X20    4 lasers, 405nm, 488nm, 561nm and 640nm with HTS

Fortessa1         4 lasers, 405nm, 488nm, 561nm and 640nm with HTS

LSR IIW            4 lasers, 405nm, 488nm, 561nm and 640nm

LSRC                3 lasers, 355nm, 488nm, and 640nm

Canto               3 lasers, 405nm, 488nm, and 640nm

FACS Verse     3 lasers, 405nm, 488nm, and 640nm

Calibur1           2 lasers, 488nm and 640nm with HTS

Calibur2           2 lasers, 488nm and 640nm 

Calibur3           2 lasers, 488nm and 640nm 

 

PC3 cell analysis and cell sorting

The institute has a new PC3 facility which houses a FACS Aria Fusion cell sorter and a FACS Verse analyzer. This equipment is available for use to internal and external researchers.

Instrument booking

Booking is completed via our online PPMS booking system.

New users can request an account via the booking system webpage.

External users must fill in the external user form via the booking system webpage.

Charges

External academic users

Two laser analyser: $35/hr

Multi laser analyser: $50/hr

Cell sorting service: $120/hr

External commercial users

Two laser analyser: $40/hr

Multi laser analyser: $60/hr

Cell sorting service: $140/hr

Internal users

Two laser analyser: $30/hr

Multi laser analyser: $45/hr

Cell sorting service: $100/hr