Professor Gordon Smyth - Bioinformatics division

Professor Gordon Smyth - Bioinformatics division

Location: 
Davis Auditorium
Start Time: 
Wed, 23/10/2019 - 1:00pm
End Time: 
Wed, 23/10/2019 - 2:00pm

Using Next Generation Sequencing to understand single cell heterogeneity, long-range enhancers and gene isoforms

Wednesday seminar​ hosted by Professor Tony Papenfuss

Professor Smyth is a statistical bioinformatician who develops statistical methods and computational algorithms for analysing genomic data. His lab has developed software tools for analysing Next Generation Sequencing data that have become very widely used around the world. He collaborates widely with biomedical scientists at the Institute and elsewhere to understand cancer and immunological diseases.

The Institute has by now about 10 years experience with high-throughput DNA sequencing and especially with the Illumina HiSeq, NextSeq and MiSeq technologies. The technologies are widely used at the WEHI to measure gene expression (via RNA-seq) and to understand the mechanisms of gene regulation (using ChIP-seq, ATAC-seq, BS-seq, Hi-C etc). The technologies rely heavily on bioinformatics tools for their use and interpretation and they have motivated the development of a huge body of bioinformatics methodology. This talk will start with a review of some of the underlying principles that have been learnt by Prof Smyth's group and which underly effective statistical analyses of Next Generation Sequencing data. The talk will go on to give examples of current projects using scRNA-seq to understand the genesis of breast cancer, using Hi-C to detect long-range enhancers and extending RNA-seq analysis methods to measure gene isoforms and detect alternative splicing.