Simon Monard & Dr Maximilien Evrard - WEHI & Doherty Institute

Simon Monard & Dr Maximilien Evrard - WEHI & Doherty Institute

Location: 
Online
Start Time: 
Tue, 19/10/2021 - 2:30pm
End Time: 
Tue, 19/10/2021 - 3:30pm

Special Lecture hosted by Dr Kelly Rogers – Centre for Dynamic Imaging

Simon Monard

Head, Flow Cytometry Facility - WEHI

Full Spectrum Cytometry

Simon Monard did his BSc Hons in University College London followed by a Masters in Immunology from Kings College London. He worked first in several London hospitals phenotyping leukaemia and lymphoma samples before moving to the Cytometry Lab at Imperial Cancer Research Fund which became Cancer Research UK now at the Crick Institute. Simon moved to Cambridge where he ran the Flow Cytometry Facility at the Department of Medicine, University of Cambridge. Next Simon moved to the Aaron Diamond Aids Research Centre in NYC running the Flow Facility and phenotyping blood from patients enrolled in clinical trials for treatment of AIDS with HAART. After a two year stint at IBMC in Porto in Portugal Simon moved to the Scottish Centre for Regenerative Medicine in Edinburgh again running the flow cytometry facility. Finally in 2012 Simon moved to WEHI here in Melbourne to head the Flow Cytometry Facility.

Conventional flow cytometers use one detector for one fluorescent dye. The inevitable spillover of light from one dye into detectors intended for other dyes is corrected for using colour compensation. Spectral flow cytometry breaks this one dye one detector paradigm and will have more detectors than fluorescent dyes and use spectral unmixing to deconvolute the data. The first commercial spectral cytometer was launched in 2014 since then several companies have released instruments which use the full spectrum approach. WEHI has two Cytek Aurora instruments which can run 43+ fluorescent dyes simultaneously.  These instruments have a number of advantages over conventional instruments which will be explored.

 

Dr Maximilien Evrard

McKenzie Postdoctoral Fellow - Doherty Institute



Resolving tissue-resident lymphocytes using spectral cytometry

Dr Maximilien Evrard is a McKenzie Postdoctoral Fellow at the Peter Doherty Institute, University of Melbourne. He completed his PhD in 2017 at the Singapore Immunology Network (SIgN, A*STAR) under Dr Lai Guan Ng, where he studied myeloid cell development in the bone marrow and identified committed neutrophil precursors using mass cytometry. Upon graduation, Maximilien took up a post-doctoral position with Prof Laura Mackay at the University of Melbourne. Here, he led a study investigating how migratory pathways impact the differentiation of tissue-resident memory T (TRM) cells and is currently developing high-dimensional protein screen approaches using spectral cytometry to elucidate TRM cell diversity across tissues. Maximilien’s research has resulted in first author publications in leading journals including Immunity and Journal of Experimental Medicine, and has been the recipient an ARC Discovery Project grant as well as several research travel awards.

Maximilien’s presentation will focus on the design of high-parameter cytometry experiments (>30 fluorescent markers) using the Cytek Aurora. He will discuss fluorochrome choices and their impact on spectral complexity, the influence of enzymatic digestion on unmixing results in multiple tissues, and will provide examples of downstream high-dimensional data analysis.

 

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Including Q&A session

 

All welcome!