Novel regulators of JAK-STAT signalling in development and disease

Novel regulators of JAK-STAT signalling in development and disease

Project details

The signalling proteins of the JAK-STAT pathway and their importance in blood formation and immune cell responses is well characterised. However, it is possible that other genes remain undiscovered that are critical for the pathway to function. Using a whole genome CRISPR/Cas9 knockout screen in a model leukaemia cell line of interleukin-6 (IL-6) activated JAK-STAT signalling, we have recently identified the histone methyltransferase Setdb1 as a novel regulator of the pathway.

The aim of this project will be to characterise the role of Setdb1 in regulating JAK-STAT signalling. Skills learned will include molecular cloning, DNA preparation, cell culture, lentivirus production, gene editing using CRISPR/Cas9, western blotting, chromatin immunoprecipitation (ChIP) and RNA-sequencing.

About our research group

The Hilton laboratory studies the development and function of blood cells. We aim to identify genes and molecular pathways that are important in regulating normal blood cell production and understand the changes that lead to blood cell disorders such as autoimmune and inflammatory diseases, leukemia and lymphoma. We work with clinicians and industry partners to find better ways of treating these illnesses.

We take a multidisciplinary approach combining cell biology, physiology, biochemistry and molecular biology, genomics, bioinformatics and computational science to understand the blood cell system and its individual lineages. We also develop software that assists biologist in visualising and interacting with large data sets.


Professor Doug Hilton

Professor Doug Hilton at the Institute
Institute Director, Division Head
Dr Andrew Jarratt profile photo
Molecular Medicine division

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